Cov teeb meem hauv Kev Tswj Xyuas Cell Viability Nyob Hauv Cell imaging System thaum imaging

Cov duab nyob hauv cell yog ib qho tseem ceeb analytical cuab tam nyob rau hauv lub chaw soj nstuam kawm txog biomedical kev qhuab qhia, xws li cell biology, neurobiology, pharmacology, thiab kev loj hlob biology. Kev yees duab ntawm cov hlwb thiab cov ntaub so ntswg (rau qhov photobleaching yog qhov teeb meem loj) feem ntau yuav tsum tau siv lub teeb pom kev zoo thiab lub sijhawm ntev; Txawm li cas los xij, cov no yuav tsum tau zam thaum kuaj cov cell nyob. Live-cell microscopy feem ntau muaj kev cuam tshuam ntawm kev tau txais cov duab zoo thiab tswj cov hlwb noj qab haus huv. Yog li ntawd, kom tsis txhob muaj lub teeb pom kev zoo thiab lub sijhawm ntev, kev daws teeb meem ntawm lub cev thiab lub cev yog feem ntau txwv hauv kev sim. Kev yees duab nyob hauv hlwb suav nrog ntau yam kev sib piv-txhim kho cov duab rau kev kho qhov muag microscopy. Kev tshawb nrhiav feem ntau siv ib qho ntawm ntau hom fluorescence microscopy, thiab qhov no feem ntau ua ke nrog cov txheej txheem teeb pom kev zoo, uas yuav tau tham hauv qab no. Kev nce qib tsis tu ncua hauv cov tswv yim kev yees duab thiab kev tsim cov fluorescent probes txhim kho lub zog ntawm txoj hauv kev no, kom ntseeg tau tias cov duab nyob hauv cell yuav tseem yog ib qho tseem ceeb hauv biology.

Ib qho kev ceeb toom tseem ceeb yog kom ntseeg tau tias cov hlwb nyob hauv qhov zoo thiab ua haujlwm ib txwm muaj thaum nyob rau ntawm lub tshuab tsom iav nrog lub teeb pom kev nyob rau hauv muaj cov hluavtaws fluorophores lossis fluorescent proteins. Cov xwm txheej nyob rau hauv uas cov hlwb tau khaws cia nyob rau theem ntawm lub tshuab tsom, txawm hais tias muaj ntau qhov sib txawv, feem ntau hais txog kev ua tiav lossis tsis ua tiav ntawm kev sim.

Ntau yam xov xwm kab lis kev cai ntawm tes yog muaj raws li qhov tshwj xeeb biochemical xav tau ntawm cov hlwb. Kab lis kev cai xov xwm muaj ntau yam xws li amino acids, vitamins, inorganic ntsev (minerals), kab kawm, nucleic acid constituents (bases thiab nucleosides), qab zib, tricarboxylic acid cycle intermediates, lipids, thiab co-enzymes. Hauv cov ntaub so ntswg kab lis kev cai, ib kauj ruam tseem ceeb yog tswj cov pa oxygen concentration, pH, buffering peev xwm, osmolarity, viscosity, thiab nto nro. Kev lag luam uas muaj cov qauv tsim tawm feem ntau suav nrog cov cim xim (xws li, phenol liab) kom pom qhov pom ntawm tus nqi pH. Cov pa roj carbon dioxide thiab bicarbonate tsis siv rau kev tswj pH yog xav tau rau yuav luag txhua kab ntawm tes. Cov hlwb yuav tsum tau coj mus rau hauv ib qho chaw uas muaj cov pa roj carbon dioxide me me (feem ntau 5-7%) nyob rau hauv incubators los tswj cov roj concentration. Rau cov duab nyob hauv cell, qhov chaw tsim nyog nrog cov pa roj carbon dioxide tuaj yeem nyuaj rau muab, thiab qhov no feem ntau yuav tsum tau tsim cov kab lis kev cai tshwj xeeb rau cov huab cua tswj. Cov kev cai pa oxygen tuaj yeem sib txawv ntawm cov kab ntawm tes, tab sis cov pa oxygen tensions ib txwm muaj rau ntau haiv neeg. Hais txog osmolarity, feem ntau ntawm cov kab ntawm tes muaj lub siab ntev rau osmotic siab, nrog kev loj hlob zoo ntawm osmolarities ntawm 260 thiab 320 milliosmolar. Thaum cov hlwb loj hlob hauv cov kab lis kev cai qhib lossis Petri cov tais diav, cov nruab nrab hypotonic tuaj yeem siv los tiv thaiv evaporation.