Nyob Cell Imaging System
Live cell imaging system yog txoj kev tshawb fawb ntawm cov cell nyob siv lub sij hawm-lapse microscopy. Nws yog siv los ntawm cov kws tshawb fawb kom tau txais kev nkag siab zoo ntawm kev ua haujlwm lom neeg los ntawm kev kawm ntawm cellular dynamics. Txij thaum ntawd los, ob peb txoj hauv kev siv tshuab microscopy tau tsim los kawm cov hlwb nyob hauv kev nthuav dav dua nrog kev siv zog tsawg. Ib hom duab tshiab uas siv quantum dots tau siv, vim tias lawv tau pom tias muaj kev ruaj khov dua. Txoj kev loj hlob ntawm holotomographic microscopy tau tsis pom zoo rau phototoxicity thiab lwm yam staining-derived tsis zoo los ntawm kev siv cov staining raws li cov cell 'refractive index.
Hauj lwm lawm
Tuam txhab Profile
Guangzhou G-Cell Technology Co., Ltd. yog lub tuam txhab thev naus laus zis tshiab tsim los ntawm kev cia siab rau Tsinghua University Shenzhen Kawm Tiav Tsev Kawm Ntawv, Southern University of Science thiab Technology, thiab South China Normal University, thiab peb tsom mus rau daim ntawv thov ntawm optical imaging technology nyob rau hauv lub teb ntawm life sciences. Rau cov chav nyob hauv cov lus qhia txog kev thov, peb tuaj yeem muab koj cov khoom siv kho qhov muag pom kev thiab cov kev daws teeb meem. Peb muaj qhov ua tiav kev sim kho qhov muag qhov chaw sim thiab ib pawg ntawm cov tub ntxhais hluas cov pob txha zoo. Raws li kev sib koom ua ke ntawm cov khoom siv hauv chav kuaj thiab kev lag luam hauv Is Taws Nem, lub tuam txhab tau cog lus los tsim lub cim tshiab ntawm cov khoom siv ntse ntse.
Vim Li Cas Xaiv Peb
Pab neeg ua haujlwm
Peb tshwj xeeb hauv kev siv thev naus laus zis kho qhov muag rau thaj tsam ntawm cell biology. Rau kev tshawb fawb ntawm tes, kev soj ntsuam thiab lwm daim ntawv thov teb.Peb muaj qhov ua tiav kev sim kho qhov muag sim platform thiab ib pawg ntawm cov tub ntxhais hluas cov pob txha zoo tshaj plaws.
Cov cuab yeej siv siab heev
Raws li kev sib koom ua ke ntawm cov khoom siv hauv chav kuaj thiab kev lag luam hauv Is Taws Nem, lub tuam txhab tau cog lus los tsim lub cim tshiab ntawm cov khoom siv ntse ntse.
Kev tshawb fawb ywj pheej thiab kev txhim kho
Raws li kev tsim kho tshiab ntawm pab pawg tshawb fawb thiab kev txhim kho muaj zog, GCell cov khoom lag luam tau txais kev ywj pheej tshawb fawb thiab kev tsim kho, kev tsim khoom ywj pheej, kev ywj pheej patents, thiab tau dhau ntau daim ntawv pov thawj xws li software monographs thiab cov qauv siv hluav taws xob patents.
Software zoo
Software tuning yog ua raws li kev siv tus cwj pwm ntawm cov neeg siv tshawb fawb tshawb fawb, thiab cov txiaj ntsig tau raug xa tawm raws li qhov xav tau ntawm cov ntawv tshawb fawb tshawb fawb thiab cov ntawv ceeb toom. Daim ntawv saib ua ntej cov ntaub ntawv tuaj yeem muab rov qab tau txhua lub sijhawm, thiab txhawb kev hloov pauv ntawm cov txiaj ntsig panoramic, uas yooj yim rau kev tshawb fawb thoob ntiaj teb.
Yam khoom
Dab tsi yog Live Cell Imaging System
Live cell imaging system yog txoj kev tshawb fawb ntawm cov cell nyob siv lub sij hawm-lapse microscopy. Nws yog siv los ntawm cov kws tshawb fawb kom tau txais kev nkag siab zoo ntawm kev ua haujlwm lom neeg los ntawm kev kawm ntawm cellular dynamics. Txij thaum ntawd los, ob peb txoj hauv kev siv tshuab microscopy tau tsim los kawm cov hlwb nyob hauv kev nthuav dav dua nrog kev siv zog tsawg. Ib hom duab tshiab uas siv quantum dots tau siv, vim tias lawv tau pom tias muaj kev ruaj khov dua. Txoj kev loj hlob ntawm holotomographic microscopy tau tsis pom zoo rau phototoxicity thiab lwm yam staining-derived tsis zoo los ntawm kev siv cov staining raws li cov cell 'refractive index.
Qhov zoo ntawm Live Cell Imaging System
Stable theem
Tau txais cov duab meej dua nrog lub phaj ruaj khov. Tsis zoo li lwm yam khoom siv, cov duab nyob ntawm tes muaj lub sijhawm ruaj khov thiab cov optics txav mus.
Siab compatibility
Tau tshaj ntau hom kab lis kev cai ntawm tes. Cov phaj zoo, tais, thiab T-flask tuaj yeem xaiv tau.
Cwj pwm thiab ua haujlwm hauv lub sijhawm
Live-cell imaging enables cov kws tshawb fawb los kawm dynamic cellular txheej txheem, tus cwj pwm thiab ua hauj lwm nyob rau hauv lub sij hawm thiab dhau sij hawm, yog li muab ib tug tiag tiag saib ntawm lom muaj nuj nqi.
Muaj peev xwm txheeb xyuas txhua lub sijhawm
Kinetic nyob-cell imaging zam qhov yuav tsum tau npaj ib qho qauv cais rau txhua lub sij hawm taw tes los soj ntsuam - ib qho qauv tuaj yeem txheeb xyuas lub sijhawm.
Yuav tsum xav txog dab tsi thaum xaiv lub tshuab kuaj kab mob zoo rau Live Cell Imaging System
Txhawm rau ua qhov kev sim nyob-cell imaging ua tiav, siv txoj hauv kev yog qhov tseem ceeb. Thaum xaiv lub tshuab ntsuas qhov tsim nyog rau koj cov duab nyob hauv cell, cov hauv qab no yuav tsum tau txiav txim siab: Cov qauv muaj peev xwm, cov duab tau nrawm (kev daws teeb meem ntawm lub sijhawm) thiab xav tau kev daws teeb meem hauv peb qhov loj me.
During live cell imaging, certain environmental conditions must be maintained to avoid detrimental physiological changes. In order to capture physiologically relevant cellular dynamics, live cell experiments require specific environmental conditions, including temperature, pH (via CO2), and humidity control. Furthermore, some experiments may even require hypoxic conditions. Modern incubation systems not only tightly control environmental conditions, they can also provide detailed data reports and alert users to temperature, gas, or humidity variations during the course of an imaging experiment. To minimize or avoid the effects of photodamage, getting the right balance between sensitive detection, accurate label separation (if using >1 daim ntawv lo) thiab qhov tsawg tshaj plaws lub teeb ntau npaum li cas rau excitation yog qhov tseem ceeb.
Rau cov kev sim ntawm tes nyob, kev tau txais kev ceev ceev feem ntau yog qhov tseem ceeb, tshwj xeeb tshaj yog rau kev kawm ntawm cov txheej txheem ceev ceev xws li kev soj ntsuam vesicle. Kev siv cov ntxaij lim dej kho qhov muag ua rau muaj kev txwv ceev vim qhov tsim nyog rau cov duab sib txuas thaum hloov cov lim lim rau txhua xim, siv los kawm txog kev sib cuam tshuam ntawm ntau yam khoom. Kev sib sau cov duab sib txuas yuav tsum tau siv sijhawm ntau dua li cov duab sib sau ua ke thiab, vim li ntawd, cov qauv nrawm nrawm tuaj yeem plam thaum tau txais, vim tias txhua xim muaj sijhawm ntev dua los ntawm ib daim duab mus rau lwm qhov. Nyob rau sab saum toj thaum qhov kev sib piv ncaj qha ntawm ob lossis ntau xim yog qhov tseem ceeb, cov cim qhia tau txav mus txawm tias ntawm tus neeg tau txais cov fluorophores, ua rau nyuaj rau kev txhais cov ntaub ntawv.
Ntau lub tshuab muaj nyob rau kom tau txais cov duab hauv 3 qhov ntev dhau sijhawm. Qhov kev xaiv ntawm lub kaw lus nyob ntawm koj qhov kev sim thiab seb qhov nrawm dua lossis tsawg dua cov qauv teeb pom kev thaum lub sijhawm thaij duab yog koj qhov tseem ceeb thaum tau txais qhov xav tau 3D daws teeb meem. Kev xaiv qhov tsim nyog tshaj plaws yog ib txwm xav kom koj xaiv qhov kev xaiv ntawm lub koob yees duab-raws li lossis confocal nyob cell imaging system, txawm li cas los xij cov kev daws teeb meem niaj hnub tuaj yeem muab ob qho tib si hauv kev sib koom ua ke.
Peb muab cov txheej txheem tshiab thiab thev naus laus zis los pab koj ua tiav koj lub hom phiaj r&d. Peb cov automated cell imagers muab cov duab zoo tshaj plaws ntawm txhua lub xov tooj ntawm tes hauv kev ua lag luam, thiab ua ke nrog kev txiav cov software suites thiab cov chaw kuaj automation daws teeb meem, xyuas kom muaj txiaj ntsig zoo tshaj plaws hauv koj daim ntawv thov.
Kev loj hlob ntawm cov kab mob (eG Ib leeg cell cloning, pov thawj ntawm monoclonality, crispr/cas9 nrhiav, transfection efficiency, cell viability xyuas, paia protein titer ntsuas, paia glycosylation ntsuas, fluorescent activated ib leeg cell cloning (fascc)). Kev tshawb nrhiav mob qog noj ntshav thiab tshuaj nrhiav pom (eG Kev Ntsuas ntawm 3d spheroids, kuaj tshuaj lom neeg, ic50 kev tshawb fawb, kev nthuav dav ntawm tes, saib xyuas apoptosis, nucleus characterization, qhov txhab kho & tsiv teb tsaws, yh2ax-dna-kev puas tsuaj, cell cycle & mitosis).
Kev tshawb fawb qia cell (eG Ips colony suav, fluorescent pluripotency kev tshawb fawb, validation ntawm proliferation thiab cell migration, cell differentiation analysis, recombinant lectin probes, cornea cell count, sirna detection, ips-cell-marker characterization). Immunology (eG b-cell thiab t-cell kev tshawb fawb, cytotoxic t-lymphocyte test, kev soj ntsuam ntawm pab t-cells thiab subsets, ua cov kev tshawb fawb cell tuag).
Kev tshawb fawb tshuaj tiv thaiv (eG Focus forming assay (ffa) for virus titer quantification, immunofluorescence foci assay (ifa) for viral infectivity, viral plaque assay, viral pathogenesis with quantifying morphological change, transduction efficiency with fluorescence coupled gene expression, cytopathic effect (cytopathic effect) ).
Lub tshuab hluav taws xob nyob hauv lub cev uas tau nruab nrog lub tshuab fluorescence siab heev thiab lub teeb pom kev zoo, autofocusing thiab lub sij hawm ntawm ntau txoj hauj lwm imaging technology rau lub phaj zoo, tais los yog T-flask. Cov txheej txheem streamlined muab kev daws teeb meem ua haujlwm yooj yim muab rau koj tag nrho cov cuab yeej koj xav tau kom tau txais cov duab zoo tshaj plaws thiab cov txiaj ntsig kev tshawb fawb raug.Qhov kev cog lus ntawm qhov tso cai tso rau hauv qhov chaw tsim khoom muab kev txhim kho cell viability vim muaj kev cuam tshuam tsawg dua hauv chav kawm. ntawm koj qhov kev sim txo cov kev pheej hmoo ntawm cellular abnormality. Analysis rau kev txheeb xyuas thiab tom qab ua tiav cov duab.
Nws yog ib lub xovtooj ntawm tes uas yooj yim haum rau hauv tus qauv CO2 incubator. Full-automated, multi-position imaging rau high-resolution tsom xam nrog lub koob yees duab motorized uas tso cai rau cov duab ntau qhov chaw mus txog 96 qhov dej. Ua kom pom tseeb ceev thiab rov tsim dua tshiab nrog txhim khu kev qha autofocusing muaj nuj nqi. Tau tshaj ntau hom kab lis kev cai ntawm tes. Cov phaj zoo (6, 12, 24, 48, 96 lub qhov dej), tais (35 hli, 60 hli, 90 hli), thiab T-flask (25 cm2, 75cm2) tuaj yeem xaiv. Nrog rau cov neeg siv-phooj ywg ua haujlwm, cov cuab yeej tshuaj ntsuam xyuas tau yooj yim xws li cov cim tsis sib xws, kev loj hlob nkhaus thiab tus pas ntsuas tau tsim rau hauv cov software suav nrog. Khaws ntau lub dav hlau focal thiab siv Z-stacking muaj nuj nqi los saib cov duab siab ntau (HDR). Stitching combines dluab rau kev tsom xam ntawm ib tug siab daws teeb meem composite duab. Qhov no ua rau kev tsom xam ntawm cov ntim loj thiab ntu.
Lub optics system mus ncig 117mm x 77mm, x thiab y axis feem, ntau cov ntsiab lus nyob rau hauv qhov kev mus ncig yuav raug ntes tau raws li lub sijhawm (sib ntus, voj voog, tag nrho lub sijhawm) los ntawm tus kws tshawb fawb.
Kev siv cov nkoj sib txawv tuaj yeem siv tau (Zoo daim phiaj, tais diav, flasks, slides). Live cell imaging system tsis muaj theem txav tau tab sis hloov, lub koob yees duab nyob hauv lub cev txav mus ntes cov duab ntawm tes hauv ntau txoj haujlwm. Qhov tseeb thiab rhiab heev fluorescence nrhiav tau yog ua tau nrog lub hard-coated optical teeb thiab LED lim nrog ntau tshaj 50, 000- teev lub neej.
Nyob rau hauv lub cell imaging system yog compact loj nrog 226(h) x 358(l) x 215(w) mm qhov twg ntau AutoLCI systems tuaj yeem haum rau hauv tus qauv CO2 incubator. Kev tswj xyuas qhov ua tau zoo ntawm cov cuab yeej ua haujlwm hauv qhov chaw kub thiab av noo yog qhov nyuaj heev. Nrog AutoLCI, koj tuaj yeem yooj yim saib xyuas cov cell nyob hauv lub incubator rau lub sijhawm ntev yam tsis muaj kev cuam tshuam rau ib puag ncig tsim nyog rau kab lis kev cai ntawm tes.
Daim ntawv thov scanning yog siv los ntes cov duab. Koj tuaj yeem saib ua ntej cov hlwb, teem sijhawm cov duab ntes, kho lub teeb thiab qhov sib txawv, thiab saib xyuas lub sijhawm lapse kev nce qib los ntawm ib qho screen intuitive. Nws suav nrog auto-focusing thev naus laus zis uas pom lub dav hlau pom tseeb ntawm cov hlwb thiab muaj qhov rov ua tau zoo heev.
Teeb meem hauv Kev Tswj Xyuas Cell Viability nyob rau hauv Cell Imaging System Thaum imaging
Cov duab nyob hauv cell yog ib qho tseem ceeb analytical cuab yeej nyob rau hauv lub chaw soj nstuam kawm biomedical kev qhuab qhia, xws li cell biology, neurobiology, pharmacology, thiab kev loj hlob biology. Kev yees duab ntawm cov hlwb thiab cov ntaub so ntswg (rau qhov uas photobleaching yog qhov teeb meem loj) feem ntau yuav tsum tau siv lub teeb pom kev zoo thiab lub sijhawm ntev; Txawm li cas los xij, cov no yuav tsum tau zam thaum kuaj cov cell nyob. Live-cell microscopy feem ntau muaj kev cuam tshuam ntawm kev tau txais cov duab zoo thiab tswj cov hlwb noj qab haus huv. Yog li ntawd, kom tsis txhob muaj lub teeb pom kev zoo thiab lub sijhawm ntev, kev daws teeb meem ntawm lub cev thiab lub cev yog feem ntau txwv hauv kev sim. Kev yees duab nyob hauv hlwb suav nrog ntau yam kev sib piv-txhim kho cov duab rau kev kho qhov muag microscopy. Kev tshawb nrhiav feem ntau siv ib qho ntawm ntau hom fluorescence microscopy, thiab qhov no feem ntau ua ke nrog cov txheej txheem teeb pom kev zoo, uas yuav tau tham hauv qab no. Kev nce qib tsis tu ncua hauv cov tswv yim kev yees duab thiab kev tsim cov fluorescent probes txhim kho lub zog ntawm txoj hauv kev no, kom ntseeg tau tias cov duab nyob hauv cell yuav tseem yog ib qho tseem ceeb hauv biology.
Ib qho kev ceeb toom tseem ceeb yog kom ntseeg tau tias cov hlwb nyob hauv qhov zoo thiab ua haujlwm ib txwm muaj thaum nyob rau ntawm lub tshuab tsom iav nrog lub teeb pom kev nyob rau hauv muaj cov hluavtaws fluorophores lossis fluorescent proteins. Cov xwm txheej nyob rau hauv uas cov hlwb tau khaws cia nyob rau theem ntawm lub tshuab tsom, txawm hais tias muaj ntau qhov sib txawv, feem ntau hais txog kev ua tiav lossis tsis ua tiav ntawm kev sim.
Ntau yam xov xwm kab lis kev cai ntawm tes yog muaj raws li qhov tshwj xeeb biochemical xav tau ntawm cov hlwb. Kab lis kev cai xov xwm muaj ntau yam xws li amino acids, vitamins, inorganic ntsev (minerals), kab kawm, nucleic acid constituents (bases thiab nucleosides), qab zib, tricarboxylic acid cycle intermediates, lipids, thiab co-enzymes. Hauv cov ntaub so ntswg kab lis kev cai, ib kauj ruam tseem ceeb yog tswj cov pa oxygen concentration, pH, buffering peev xwm, osmolarity, viscosity, thiab nto nro. Kev lag luam uas muaj cov qauv tsim tawm feem ntau suav nrog cov cim xim (xws li, phenol liab) kom pom qhov pom ntawm tus nqi pH. Cov pa roj carbon dioxide thiab bicarbonate tsis siv rau kev tswj pH yog xav tau rau yuav luag txhua kab ntawm tes. Cov hlwb yuav tsum tau coj mus rau hauv ib qho chaw uas muaj cov pa roj carbon dioxide me me (feem ntau 5-7%) nyob rau hauv incubators los tswj cov roj concentration. Rau cov duab nyob hauv cell, qhov chaw tsim nyog nrog cov pa roj carbon dioxide tuaj yeem nyuaj rau muab, thiab qhov no feem ntau yuav tsum tau tsim tshwj xeeb cov kab lis kev cai rau cov huab cua tswj. Cov kev cai pa oxygen tuaj yeem sib txawv ntawm cov kab ntawm tes, tab sis cov pa oxygen tensions ib txwm muaj rau ntau haiv neeg. Hais txog osmolarity, feem ntau ntawm cov kab ntawm tes muaj lub siab ntev rau osmotic siab, nrog kev loj hlob zoo ntawm osmolarities ntawm 260 thiab 320 milliosmolar. Thaum cov hlwb loj hlob hauv cov kab lis kev cai qhib lossis Petri cov tais diav, cov tshuaj hypotonic tuaj yeem siv los tiv thaiv evaporation.
Yuav Ua Li Cas Live Cell Imaging System Ua Haujlwm?
Hauv kev yees duab ntawm tes nyob, cov hlwb muaj sia nyob tau pom nyob rau ib lub sijhawm nyob rau hauv lub xov tooj ntawm tes imaging microscope. Txhawm rau tso cai rau kev siv lub xov tooj ntawm tes ua haujlwm ua haujlwm, niaj hnub no cov kev daws teeb meem nyob ntawm tes feem ntau suav nrog kev tshawb fawb lub tshuab hluav taws xob tag nrho, suav nrog lub koob yees duab microscope thiab cov software tshwj xeeb los tsim thiab khiav qhov kev sim nrog rau kev txheeb xyuas cov ntaub ntawv. Cov duab ntawm ib qhov chaw saib lossis txawm tias tag nrho cov qauv hauv cheeb tsam raug kaw ua ntu zus tom qab qee lub sijhawm cov ntsiab lus nyob rau lub sijhawm ntev dua. Txhawm rau kom cov hlwb hauv lub cev muaj zog thoob plaws qhov kev sim, cov kab ke hauv cov duab nyob hauv lub cev feem ntau yog nruab nrog cov chaw tsim hluav taws xob kom tswj tau qhov kub thiab txias, av noo thiab CO2 concentration. Nws yog ib qho tseem ceeb uas cov kev txwv no tuaj yeem hloov kho raws li cov kev xav tau ntawm lub hlwb thiab lawv tuaj yeem khaws cia nyob rau theem tas mus li rau tag nrho lub sijhawm ntawm kev sim.
Cells tuaj yeem ua duab nrog cov duab sib txawv xws li brightfield microscopy, txhawb nqa piv txwv los ntawm kev sib piv theem. Tsis tas li ntawd, ob peb lub xov tooj ntawm tes imaging cov tswv yim tau hloov zuj zus los ntawm kev siv cov xov tooj ntawm tes fluorescent dyes kom muaj peev xwm txheeb xyuas cov hlwb uas txaus siab thiab tseem xaiv los saib xyuas kev loj hlob, kev sib txawv lossis kev muaj peev xwm ntawm cov hlwb. Yog li, nyob hauv cell fluorescence microscopy yog ib qho cuab yeej pab uas tuaj yeem pom ntau cov ntaub ntawv ntxiv ntawm tus kheej lub hlwb. Live cell super daws teeb meem microscopy lossis 3D nyob cell imaging muab qhov tob ntxiv thiab kev nkag siab rau hauv kev tsom xam ntawm cov cell nyob.
Cov duab kaw tuaj yeem qhib, saib thiab txheeb xyuas siv cov khoom siv ntawm tes tsom xam software. Cov duab ntawm ib leeg tuaj yeem hloov mus ua cov yeeb yaj kiab nyob hauv xov tooj thiab cov software algorithms muab cov ncauj lus kom ntxaws txheeb xyuas ntawm cov hlwb nyob rau lub sijhawm xws li cov kab mob ntawm cov cell tsiv teb tsaws. Lub sij hawm yog li ntawd tsis yog lwm qhov me me hauv kev yees duab ntawm tes, tab sis nws tso cai rau pom cov txheej txheem uas peb yuav tsis muaj peev xwm nkag siab.
Peb lub Hoobkas
Guangzhou G-Cell Technology Co., Ltd. yog lub tuam txhab thev naus laus zis tshiab tsim los ntawm kev cia siab rau Tsinghua University Shenzhen Kawm Tiav Tsev Kawm Ntawv, Southern University of Science thiab Technology, thiab South China Normal University, thiab peb tsom mus rau daim ntawv thov ntawm optical imaging technology nyob rau hauv lub teb ntawm life sciences. Rau cov chav nyob hauv cov lus qhia txog kev thov, peb tuaj yeem muab koj cov khoom siv kho qhov muag pom kev thiab cov kev daws teeb meem. Peb muaj qhov ua tiav kev sim kho qhov muag qhov chaw sim thiab ib pawg ntawm cov tub ntxhais hluas cov pob txha zoo. Raws li kev sib koom ua ke ntawm cov khoom siv hauv chav kuaj thiab kev lag luam hauv Is Taws Nem, lub tuam txhab tau cog lus los tsim lub cim tshiab ntawm cov khoom siv ntse ntse.
FAQ
Cim npe nrov: Tuam Tshoj nyob cell imaging system manufacturers, lwm tus neeg
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