Yuav xav txog dab tsi thaum xaiv Qhov Zoo Tshaj Plaws Microscope Rau Live Cell Imaging System

Txhawm rau ua qhov kev sim nyob-cell imaging ua tiav, siv txoj hauv kev yog qhov tseem ceeb. Thaum xaiv lub tshuab ntsuas qhov tsim nyog rau koj cov duab nyob hauv cell, cov hauv qab no yuav tsum tau txiav txim siab: Cov qauv muaj peev xwm, cov duab tau nrawm (kev daws teeb meem ntawm lub sijhawm) thiab xav tau kev daws teeb meem hauv peb qhov loj me.

During live cell imaging, certain environmental conditions must be maintained to avoid detrimental physiological changes. In order to capture physiologically relevant cellular dynamics, live cell experiments require specific environmental conditions, including temperature, pH (via CO2), and humidity control. Furthermore, some experiments may even require hypoxic conditions. Modern incubation systems not only tightly control environmental conditions, they can also provide detailed data reports and alert users to temperature, gas, or humidity variations during the course of an imaging experiment. To minimize or avoid the effects of photodamage, getting the right balance between sensitive detection, accurate label separation (if using >1 daim ntawv lo) thiab qhov tsawg tshaj plaws lub teeb ntau npaum li cas rau excitation yog qhov tseem ceeb.

Rau cov kev sim ntawm tes nyob, kev tau txais kev ceev ceev feem ntau yog qhov tseem ceeb, tshwj xeeb tshaj yog rau kev kawm ntawm cov txheej txheem ceev ceev xws li kev soj ntsuam vesicle. Kev siv cov ntxaij lim dej kho qhov muag ua rau muaj kev txwv ceev vim qhov tsim nyog rau cov duab sib txuas thaum hloov cov lim lim rau txhua xim, siv los kawm txog kev sib cuam tshuam ntawm ntau yam khoom. Kev sib sau cov duab sib txuas yuav tsum tau siv sijhawm ntau dua li cov duab sib sau ua ke thiab, vim li ntawd, cov qauv nrawm nrawm tuaj yeem plam thaum tau txais, vim tias txhua xim muaj sijhawm ntev dua los ntawm ib daim duab mus rau lwm qhov. Nyob rau sab saum toj thaum qhov sib piv ncaj qha ntawm ob lossis ntau xim yog qhov tseem ceeb, cov teeb liab yuav tau txav mus los ntawm tus neeg tau txais cov fluorophores, ua rau muaj kev cuam tshuam rau kev txhais cov ntaub ntawv.

Ntau yam thev naus laus zis muaj nyob rau kom tau txais cov duab hauv 3 qhov ntev dhau sijhawm. Qhov kev xaiv ntawm lub kaw lus nyob ntawm koj qhov kev sim thiab seb qhov nrawm dua lossis tsawg dua cov qauv teeb pom kev thaum lub sijhawm thaij duab yog koj qhov tseem ceeb thaum tau txais qhov xav tau 3D daws teeb meem. Kev xaiv qhov tsim nyog tshaj plaws yog ib txwm xav kom koj xaiv qhov kev xaiv ntawm lub koob yees duab-raws li lossis confocal nyob cell imaging system, txawm li cas los xij cov kev daws teeb meem niaj hnub tuaj yeem muab ob qho tib si hauv kev sib koom ua ke.